A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect

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9.Biotechnology Principals and Process

medium

A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment ?

Option A

Option B

Option C

Option D

Solution

It is very difficult to distinguish transformant and non-tranformants. Selectable markers help in selecting the transformed plasmid. The experiment is affected as one can make sure the presence of recombinant plasmid in the vector.

Standard 12

Biology

Which of the following key factors, makes the plasmid, the vector in genetic engineering?

medium

$A$ – amp$^R$ has recognition sites for Pvu $I$.

$R$ – tet$^R$ gene has not recognition site for Hind $III$.

medium

$I.$ Ori also controls the copy number of thelinked $DNA$.

$II.$ If a foreign $DNA$ ligates at the Bam Hl site oftetracycline resistance gene in the vector $pBR322$, the recombinant plasmid loses the tetracycline resistance due to insertion offoreign $DNA$.

Choose regarding the above statements.

medium

Match the columns :

Column – $I$ Column – $II$

$(a)$ Ligase $(i)$ It removes nucleotide from $DNA$ end.

$(b)$ Endonuclease $(ii)$ Connects parts of $DNA$

$(c)$ Exonuclease $(iii)$ Removes phosphates of $DNA$

$(d)$ $DNA$ Polymerase $(iv)$ It produces sticky ends in $DNA$

$(v)$ Useful to get repeated amplification

medium

Restriction in Restriction endonuclease enzyme refers to

medium

Column – $I$	Column – $II$
$(a)$ Ligase	$(i)$ It removes nucleotide from $DNA$ end.
$(b)$ Endonuclease	$(ii)$ Connects parts of $DNA$
$(c)$ Exonuclease	$(iii)$ Removes phosphates of $DNA$
$(d)$ $DNA$ Polymerase	$(iv)$ It produces sticky ends in $DNA$
	$(v)$ Useful to get repeated amplification

A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment ?

Solution

Similar Questions

Which of the following key factors, makes the plasmid, the vector in genetic engineering?

$A$ – amp$^R$ has recognition sites for Pvu $I$. $R$ – tet$^R$ gene has not recognition site for Hind $III$.

$I.$ Ori also controls the copy number of thelinked $DNA$. $II.$ If a foreign $DNA$ ligates at the Bam Hl site oftetracycline resistance gene in the vector $pBR322$, the recombinant plasmid loses the tetracycline resistance due to insertion offoreign $DNA$. Choose regarding the above statements.

Restriction in Restriction endonuclease enzyme refers to

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$A$ – amp$^R$ has recognition sites for Pvu $I$.

$R$ – tet$^R$ gene has not recognition site for Hind $III$.

$I.$ Ori also controls the copy number of thelinked $DNA$.

$II.$ If a foreign $DNA$ ligates at the Bam Hl site oftetracycline resistance gene in the vector $pBR322$, the recombinant plasmid loses the tetracycline resistance due to insertion offoreign $DNA$.

Choose regarding the above statements.