A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuc

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9.Biotechnology Principals and Process

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A recombinant $DNA$ molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant $DNA$. How does this affect the next step in the experiment, i.e. bacterial transformation ?

Option A

Option B

Option C

Option D

Solution

The experiment will not likely to be affected as recombinant $DNA$ molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of $DNA.$

Standard 12

Biology

In gel electrophoresis, the separated bands of $DNA$ are cut out and extracted from the gel piece. This step is called

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$DNA$ can be introduced into any cell by:

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Cutting of a piece of $DNA$ from a plasmid was done with the help of $…A…$ enzymes, popularly known as $…B….$ Here $\mathrm{A}$ and $\mathrm{B}$ can be

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Normal $E.$ coli cells carry resistance against which of the following antibiotics?

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Restriction endonucleases

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A recombinant $DNA$ molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant $DNA$. How does this affect the next step in the experiment, i.e. bacterial transformation ?

Solution

Similar Questions

In gel electrophoresis, the separated bands of $DNA$ are cut out and extracted from the gel piece. This step is called

$DNA$ can be introduced into any cell by:

Cutting of a piece of $DNA$ from a plasmid was done with the help of $…A…$ enzymes, popularly known as $…B….$ Here $\mathrm{A}$ and $\mathrm{B}$ can be

Normal $E.$ coli cells carry resistance against which of the following antibiotics?

Restriction endonucleases

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