$A$ : Restriction enzymes belong to a larger class of enzymes called nucleases.

$R$ : Each restriction enzyme recognises a specific palindromic nucleotide sequence in the $DNA$.

The flowchart given below represent the process of recombinant technology. Identify $A$ and $D$

$I.$ DNA being a hydrophilic molecule cannot pass through cell membranes

$II.$ The bacteria should be made competent to accept the $DNA$ molecule

The correct option regarding the above statements is

An antibiotic resistance gene in a vector usually helps in the selection of

Special sequence in the $DNA$ recognized by restriction endonuclease is called