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Consider the following statements
$I.$ Recombinant $DNA$ technology popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic material by man in vitro
$II. pBR322$ is the first artificial cloning vector developed in $1977$ by Boliver and Rodriquez from $E$. coli plasmid
$III.$ Restriction enzymes belong to a class of enzymes called nucleases
Which of the statements given above are correct?
$I \;and \;II$
$I \;and\; III$
$II \;and\; III$
$I, II\; and\; III$
Solution
Genetic engineering is a branch of biotechnology, which deals with the manipulation of genetic material by man. The technique of genetic engineering includes.$(i)$ formation of 'recombinant $DNA$'.$(ii)$ use of gene cloning. $(iii)$ gene transfer. $1. pBR \;322$ was the first artificial cloning vector constructed in $1977$ by Boliver and Rodriguer. It is widely used in gene cloning experiments. $2.$ Restriction enzymes belongs to a class of enzymes called nucleases