Escherichia coli fully labelled with 15 mathrm{~N} is allowed to grow in 14 mathrm{~N} medium. two s

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5.Molecular Basis of Inheritance

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Escherichia coli fully labelled with $15 \mathrm{~N}$ is allowed to grow in $14 \mathrm{~N}$ medium. The two strands of $DNA$ molecule of the first generation bacteria have

different density and do not resemble with their parent $DNA.$

different density but resemble with their parent $DNA.$

same density and resemble with their parent $DNA.$

same density but do not resemble with their parent $DNA.$

Solution

Messelson and Stahl $(1958)$ cultured (Escherichia coli) bacteria in a culture medium containing $15 \mathrm{~N}$. After these had been replicated for a few generations in the medium both the strands of their DNA contained $15 \mathrm{~N}$ as constituents of purines and pyrimidines. When these bacteria with $15 \mathrm{~N}$ were transferred in cultural medium containing $14 \mathrm{~N}$, it was found that $DNA$ separated from fresh generation of bacteria possesses one strand heavier than the other. The heavier strand represents the parental strand and lighter one is the new one synthesized from the culture indicating semi conservative mode of $DNA$ replication.

Standard 12

Biology

Given below are two statements :

Statement $I:$

DNA polymerases catalyse polymerisation only in one direction, that is $5^{\prime} \rightarrow 3^{\prime}$

Statement $II:$

During replication of $DNA$, on one strand the replication is continuous while on other strand it is discontinuous.

In the light of the above statements, choose the correct answer from the options given below :

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(NEET-2022)

Escherichia coli fully labelled with $15 \mathrm{~N}$ is allowed to grow in $14 \mathrm{~N}$ medium. The two strands of $DNA$ molecule of the first generation bacteria have

Solution

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Escherichia coli fully labelled with $15 \mathrm{~N}$ is allowed to grow in $14 \mathrm{~N}$ medium. The two strands of $DNA$ molecule of the first generation bacteria have

Solution

Similar Questions

Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl’s experiment.

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Which one of the following correctly represents the manner of replication of $DNA$ ?

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