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Following enzymes/chemical/technique are used in the process of gel electrophoresis
$I.$ sample $DNA$ is cut into fragments
$II.$ restriction endonucleases
$III.$ agarose gel
$IV.$ ethidium bromide
$V.$ UV-radiation
$VI.$ elution
Mark the correct sequence of their use
$I, II, III, VI, V\; and\; IV$
$I, II, III, IV, V\; and \;VI$
$IV, V, VI, I, II \;and\; III$
$I, II, IV, V, VI\; and \;III$
Solution
In gel electrophoresis a molecule of $DNA$ can be cut into fragments by the enzyme restriction endonuclease. $DNA$ fragments move towards the anode according to their molecular size through the agarose gel. The separated $DNA$ fragments can be observed only after staining them with a solution of ethidium bromide. The bright orange coloured bands of $DNA$ can be seen only under $UV$ light. These bands of $DNA$ fragments are cut out from the gel and extracted by using convenient technique. This step is called elution
