Gene of interest was cloned at site Sal I in pBR322 . recombinant plasmid will exhibit susceptibilit

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9.Biotechnology Principals and Process

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Gene of interest was cloned at site Sal I in $pBR322$. The recombinant plasmid will exhibit susceptibility to

A

Tetracycline

B

Tetracycline and Ampicillin

C

Ampicillin only

D

Broad spectrum of antibiotics

Solution

It has two antibiotic resistance genes ($amp^R$ and $tet^R$)
If a gene of interest is cloned at site sal I in $pBR322$, the so formed recombinant plasmid will exihibit susceptibility to tetracycline only as tetracycline resistant gene gets inactivated due to insertion.

Standard 12

Biology

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(NEET-2022)