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5.Molecular Basis of Inheritance
normal
Genes of interest can be selected from a genomic library by using
A
cloning vectors
B
$DNA$ probes
C
gene targets
D
restriction enzymes
(NEET-2013)
Solution
(b) : Gene bank or genomic library is a complete collection of cloned $DNA$ fragments which comprises the entire genome of an organism. Molecular probes are small $DNA$ segments that are used to detect the presence of complementary sequences in nucleic acid samples in genomic library. These are usually formed of $200-500$ nucleotide sequences. These segments or probes are labelled either with radioactive or with nonradioactive compound. Probes with $DNA$ sequence complementary to the gene to be isolated are used. They bind with the desired gene, making it visible and help in isolating it from the library.
Standard 12
Biology
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