Give types and functions of Nuclease Enzyme.

$I.$ Copy number is defined as the number ofcopies of plasmid present in a cell.

$II.$ It varies from $15-100$ copies per cell. Choose regarding the above statements.

How is copy number of the plasmid vector related to yield of recombinant protein ?

For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
 

It is called sticky ends.