The critical research areas of biotechnology are

$I$. providing best catalyst as improved organism, usually a microbes or pure enzyme

$II$. creating optimal conditions by engineering for a catalyst to act

$III$. down stream processing technologies

$IV$. Multiple Ovulation Transfer Technology $(MOET)$

Which of the statements given above the correct?

Terramycin is obtained from

$A$ : Some nations are developing laws to prevent unauthorised exploiation of their bioresources and traditional knowledge.
$R$ : Brazzein, a protein obtained from West African plant, was isolated, sequenced and patented in $U.S.A.$

$A$ : Production of hirudin from transgenic Brassica required use of synthetic gene.

$R $ : Eukaryotic genes have intron sequences that need to be spliced out.

Consult internet and find out how to make orally active protein pharmaceutical. What is the major problem to be encountered?