A tumor inducing plasmid widely used in the production of transgenic plants is that of

The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$ ' and ' $Y$ genes:

$I.$ Ori also controls the copy number of thelinked $DNA$.

$II.$ If a foreign $DNA$ ligates at the Bam Hl site oftetracycline resistance gene in the vector $pBR322$, the recombinant plasmid loses the tetracycline resistance due to insertion offoreign $DNA$.

Choose regarding the above statements.

Assertion : Insertion of recombinant $DNA$ within the coding sequence of $\beta -$ galactosidase results in colourless colonies.

Reason : Presence of insert results in inactivation of enzyme $\beta -$ galactosidase known as insertional inactivation.

Select the correct pair.