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9.Biotechnology Principals and Process
medium
Name the five key tools for accomplishing the tasks of recombinant $\rm {DNA}$ technology. Also mention the functions of each tool.
Option A
Option B
Option C
Option D
Solution
Restriction endonucleases : This enzyme is for cutting the desired $DNA $ at desired places.
Gel electrophoresis : For separating the desired $DNA$ fragments.
$\Rightarrow \quad$ Ligase enzyme : This enzyme is used for creating recombinant $DNA$ molecule.
– $DNA$ delivery system : like electroporation, microinjection, gene gun method.
Competent host (usually bacteria/yeast) : Vectors like bacteria and yeast to take up recombinant $DNA$
Standard 12
Biology
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