9.Biotechnology Principals and Process
medium

Significance of treating bacterial cells with calcium chloride before transformation is to facilitate

A

Binding of $DNA$ to the cell surface

B

Uptake of $DNA$ through membrane transport proteins

C

Uptake of $DNA$ by creating transient pores in the bacterial cell wall

D

Expression of antibiotic resistance gene

Solution

Since $DNA$ is a hydrophilic molecule, it cannot pass through cell membranes. In order to force bacteria to take up the plasmid, the bacterial cells must first be made 'competent' to take up $DNA$. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which $DNA$ enters the bacterium through pores in its cell wall. Possibly, calcium chloride causes the $DNA$ to precipitate onto the outside of the cells or it may improve $DNA$ binding.

Standard 12
Biology

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