First step in Southern Blot technique

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5.Molecular Basis of Inheritance

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What is the first step in the Southern Blot technique

Denaturation of $DNA$ on the gel for hybridization with specific probe

Production of a group of genetically identical cells

Digestion of $DNA$ by restriction enzyme

Isolation of $DNA$ from a nucleated cell such as the one from the scene of crime

Solution

(d) The first step in southern blot technique is isolation of $DNA$ from a nucleated cell and then these isolated $DNA$ sequences are transferred to a nylon or nitrocellulose sheet placed over the gel.

Standard 12

Biology

Would it be appropriate to use $DNA$ probes such as $VNTR$ in $DNA$ finger printing of a bacteriaphage ?

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Which one of the following techniques has helped to solve many mysteries involving murders, robberies and rapes?

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$DNA $ fingerprinting method is very useful for

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Arrange the various steps of $DNA$ fingerprinting technique in the correct order

$(I)$ Separation of $DNA$ fragment by electrophoresis.

$(II)$ Digestion of $DNA$ by restriction endonucleases.

$(III)$ Hybridization using labeled $VNTR$ probe

$(IV)$ Isolation of $DNA$.

$(V)$ Detection of hybridized $DNA$ fragments by autoradiography.

$(VI)$ Transferring of separated $DNA$ fragment to synthetic membranes.

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$A$ : Unknown $DNA$ after hybridization with $VNTR$ probe, the autoradiogram gives many bands of differing sizes in $DNA$ profiling.
$R$ : These bands represents $DNA$ fingerprint of organism

medium

What is the first step in the Southern Blot technique

Solution

Similar Questions

Would it be appropriate to use $DNA$ probes such as $VNTR$ in $DNA$ finger printing of a bacteriaphage ?

Which one of the following techniques has helped to solve many mysteries involving murders, robberies and rapes?

$DNA $ fingerprinting method is very useful for

$A$ : Unknown $DNA$ after hybridization with $VNTR$ probe, the autoradiogram gives many bands of differing sizes in $DNA$ profiling. $R$ : These bands represents $DNA$ fingerprint of organism

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$A$ : Unknown $DNA$ after hybridization with $VNTR$ probe, the autoradiogram gives many bands of differing sizes in $DNA$ profiling.
$R$ : These bands represents $DNA$ fingerprint of organism