What modification is done on Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning ve

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9.Biotechnology Principals and Process

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What modification is done on the $Ti$ plasmid of Agrobacterium tumefaciens to convert it into a cloning vector ?

Option A

Option B

Option C

Option D

Solution

The tumor inducing ($Ti$) plasmid of Agrobacterium tumifaciens has now been modified (disarmed). into a cloning vector. It is not pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants.

Standard 12

Biology

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A plasmid

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Which of the following statement is not correct regarding Eco $RI$ restriction endonuclease enzyme?

$I.$ Eco. $RI$ restriction endonuclease enzyme is isolated from Escherichia coli $RY13$

$II.$ Its recognition sequence is $5^{\prime}-$ $GAATTC – 3 '$

$3^{\prime}-C T T A A G-5^{\prime}$

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$3^{\prime}-C-T-T-A-A-G-5^{\prime}$

$III.$ Its site of cleavage is

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The specific palindromic sequence which is recognized by $EcoRI$ is :

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The "Ti plasmid" of Agrobacterium tumefaciens stands for

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Gene for cloning may be chemically synthesized:

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