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Which of the following shows correct chronological order of the events occurring during callus culture?
Callus $\rightarrow$ Cell division $\rightarrow$ Explant $\rightarrow$ Addition of cytokinin $\rightarrow$ Cells acquire merstematic property
Explant $\rightarrow$ Callus $\rightarrow$ Cell division $\rightarrow$ Addition of cytokinin $\rightarrow$ Cells acquire meristematic property
Explants $\rightarrow$ Cell division $\rightarrow$ Callus $\rightarrow$ Addition of cytokinin $\rightarrow$ Cells acquire meristematic property
Callus $\rightarrow$ Explant $\rightarrow$ Cell division $\rightarrow$ Addition of cytokinin $\rightarrow$ Cells acquire meristematic property
Solution
In callus culture, cell division in explant forms a callus. Callus is irregular unorganized and undifferentiated mass of actively dividing cells. Darkness and solid medium gelled by agar stimulates callus formation. The culture medium contains growth regulators auxin $2,4- D$ and often a cytokinin like $BAP$. Both of these growth regulators stimulate meristematic property in callus.
