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9.Biotechnology Principals and Process
medium
Which one of the following represents a palindromic sequence in $DNA$ ?
A
$5' - GAATTC - 3'$
$3' - CTTAAG - 5'$
B
$5' - CCAATG - 3'$
$3' - GAATCC - 5'$
C
$5' - CATTAG - 3'$
$3' - GATAAC - 5'$
D
$5' - GATACC - 3'$
$3' - CCTAAG - 5'$
(AIIMS-2014)
Solution
A palindromic sequence is a nucleic acid sequence ( $DNA$ or $RNA$ ) that is the same whether read $5'$ (five-prime) to $3'$ (three prime) on one strand or $5'$ to $3'$ on the complementary strand with which it forms a double helix.
$5 – GAATTC – 3$
$3 – CTTAAG – 5$
It is a palindromic sequence of $DNA$ cut by restriction enzyme $EcoRI$.
Standard 12
Biology