9.Biotechnology Principals and Process
medium

Would you choose an exonuclease while producing a recombinant $DNA$ molecule ?

Option A
Option B
Option C
Option D

Solution

No, as exonuclease acts on the free ends of linear $DNA$ molecule. Therefore, instead of producing $DNA$ fragments with sticky ends, it will shorten or completely degrade the $DNA$ fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.

Standard 12
Biology

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