A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining gel with ethidium

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9.Biotechnology Principals and Process

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A mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no $DNA $ bands were observed. What could be the reason ?

Option A

Option B

Option C

Option D

Solution

The reasons are as follows : $(i)$ $DNA$ sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded. $(ii)$ Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where $DNA$ sample is loaded). Since $DNA$ molecules are-negatively charged, they move towards anode and hence move out of the gel instead of moving into the matrix of gel. $(iii)$ Ethidium bromide was not added at all or was not added in sufficient concentration and so $DNA$ was not visible.

Standard 12

Biology

Which one of the following palindromic base sequences in $DNA$ can be easily cut at about the middle by some particular restriction enzyme?

medium

(AIIMS-2016)

The presence of a chromogenic substrategives………… ……coloured colonies

medium

Match the column

Substrate Enzyme

$(A)$ Ribonucleotide $(i)$ Chitinase

$(B)$ Chitin $(ii)$ Cellulase

$(C)$ Cellulose $(iii)$ Ribonuclease

medium

(AIIMS-2019)

Separation of DNA, fragments is done by a technique known as :

easy

(NEET-2022)

In gel electrophoresis, the separated bands of $DNA$ are cut out and extracted from the gel piece. This step is called

medium

Substrate	Enzyme
$(A)$ Ribonucleotide	$(i)$ Chitinase
$(B)$ Chitin	$(ii)$ Cellulase
$(C)$ Cellulose	$(iii)$ Ribonuclease

A mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no $DNA $ bands were observed. What could be the reason ?

Solution

Similar Questions

Which one of the following palindromic base sequences in $DNA$ can be easily cut at about the middle by some particular restriction enzyme?

The presence of a chromogenic substrategives………… ……coloured colonies

Match the column Substrate Enzyme $(A)$ Ribonucleotide $(i)$ Chitinase $(B)$ Chitin $(ii)$ Cellulase $(C)$ Cellulose $(iii)$ Ribonuclease

Separation of DNA, fragments is done by a technique known as :

In gel electrophoresis, the separated bands of $DNA$ are cut out and extracted from the gel piece. This step is called

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Match the column

Substrate Enzyme

$(A)$ Ribonucleotide $(i)$ Chitinase

$(B)$ Chitin $(ii)$ Cellulase

$(C)$ Cellulose $(iii)$ Ribonuclease