Agarose is a natural polymer extracted from

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9.Biotechnology Principals and Process

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Agarose is a natural polymer extracted from

A

Sea weeds

B

Kelps

C

Algae

D

Moss

Solution

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Standard 12

Biology

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A mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no $DNA $ bands were observed. What could be the reason ?

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Match the following column.

Column $I$ Column $II$

$A$. Recombinant $DNA$ $1$. Sea weeds

$B$. Gel electrophoresis $2$. DNA staining

$C$. Ethidium bromide $3$. Plasmid DNA that hasincorporated human DNA

$D$. Agarose $4$. Process by which DNA fragments are separated based on their size

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For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

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Study the given figure carefully and select the correct statements regarding this

$I.$ It represents typical agarose gel electrophoresis which showing differential migration of $DNA$ fragments

$II.$ Lane $1$ contains undigested $DNA$ fragments

$III.$ Lanes $2$ to $4$ contains digested $DNA$ fragment

$IV.$ Smallest $DNA$ bands are present at ( $A$ ) position and largest $DNA$ bands are present at ( $B$ ) position

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Consider the following statements

$I.$ A soil inhabiting plant bacterium, Agrobacteriumtumefaciens, a pathogen of several dicot plants is able to transfer a piece of $DNA$ known as $T-DNA$

$II.$ The T-DNA causes tumours

$III.$ Tumour formation induced by $Ti-$ plasmid

Which of the statements given above are correct?

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