Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells b

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9.Biotechnology Principals and Process

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Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign $DNA$ in addition to a selectable marker?

Option A

Option B

Option C

Option D

Solution

A reporter enzyme can be used to differentiate transformed cells by tracking down the activity of its co-responding genes (receptor gene). For e.g., ( $3$ – galactosidase ($Lac$ $Z$ ) activity is not found in transformed cells so that they appear white in colour. The others, which appear blue in colour, indicate that cells do not carry foreign $DNA$.

Standard 12

Biology

An antibiotic resistance gene in a vector helps in selection of

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The most important feature in a plasmid to be used as a vector is

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Which of the following is not a method of introducing alien $DNA$ into host cells?

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$A$ : $DNA$ ligase plays an important role in recombinant $DNA$ technology.

$R$ : The linking of antibiotic resistance gene with plasmid vector became possible by enzyme $DNA$ ligase.

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In the nomenclature of enzyme restrictionendonuclease the roman number indicates

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Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign $DNA$ in addition to a selectable marker?

Solution

Similar Questions

An antibiotic resistance gene in a vector helps in selection of

The most important feature in a plasmid to be used as a vector is

Which of the following is not a method of introducing alien $DNA$ into host cells?

$A$ : $DNA$ ligase plays an important role in recombinant $DNA$ technology. $R$ : The linking of antibiotic resistance gene with plasmid vector became possible by enzyme $DNA$ ligase.

In the nomenclature of enzyme restrictionendonuclease the roman number indicates

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$A$ : $DNA$ ligase plays an important role in recombinant $DNA$ technology.

$R$ : The linking of antibiotic resistance gene with plasmid vector became possible by enzyme $DNA$ ligase.