Give scientific reasons : In recombinant DNA technology use of endonuclease is more proper than exon

English

Gujarati

Hindi

9.Biotechnology Principals and Process

medium

Give scientific reasons : In recombinant $DNA$ technology the use of endonuclease is more proper than exonuclease.

Option A

Option B

Option C

Option D

Solution

In recombinant $DNA$ technology the fragment of desired $DNA$ is either joined with vector or by cutting copies are prepared. For that cutting of vector and desired $DNA$ is essential. Endonuclease does cutting in such a way that sticky ends are produced inside $DNA$. While Exonuclease remove end terminals and produce blunt ends but sticky ends are necessary to connect a piece of desired $DNA$ with vector. Therefore endonuclease is more proper enzyme than exonuclease.

Standard 12

Biology

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.

Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase

medium

(AIIMS-2009)

The cutting of $DNA$ by restriction endonucleasesresults in the fragments of $DNA$. These fragments can be separated by a technique known as ……

medium

Normal $E.$ coli cells carry resistance against which of the following antibiotics?

medium

Choose the correct pair from the following

medium

(NEET-2020)

Describe the role of Agrobacterium tumefaciens in transforming a plant cell.

medium

Give scientific reasons : In recombinant $DNA$ technology the use of endonuclease is more proper than exonuclease.

Solution

Similar Questions

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends. Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase

The cutting of $DNA$ by restriction endonucleasesresults in the fragments of $DNA$. These fragments can be separated by a technique known as ……

Normal $E.$ coli cells carry resistance against which of the following antibiotics?

Choose the correct pair from the following

Describe the role of Agrobacterium tumefaciens in transforming a plant cell.

Start a Free Trial Now

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.

Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase