Restriction endonucleases are enzymes which

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9.Biotechnology Principals and Process

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Restriction endonucleases are enzymes which

Make cuts at specific positions within the $DNA$ molecule

Recognize a specific nucleotide sequence for binding of $DNA$ ligase

Restrict the action of the enzyme $DNA$ polymerase

Remove nucleotides from the ends of the $DNA$ molecule

Solution

Restriction endonuclease recognizes a specific $DNA$ base sequence (recognition sequence, recognizion site, restriction sequence or restriction site having palindromic sequence) and cleaves both the strands of $DNA$ at or near that site. The enzyme cuts the $DNA$, generating restriction fragments with overhanging ends or blunt ends.

Standard 12

Biology