Role of DNA ligase in construction of a recombinant DNA molecule is

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9.Biotechnology Principals and Process

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The role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is

Formation of phosphodiester bond between two $DNA$ fragments

Formation of hydrogen bonds between sticky ends of $DNA$ fragments

Ligation of all purine and pyrimidine bases

None of the above

Solution

The role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is formation of phosphodiester bond between two $DNA$ fragments. $DNA$ ligase help in sealing gaps in $DNA$ fragments. Therefore, they act as a molecular glue. In $1969$ Har Govind Khorana discovered $DNA$ ligase in $T_{4}$-bacteriophage

Standard 12

Biology

The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$ ' and ' $Y$ genes:

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(NEET-2024)

A plasmid $DNA$ and a linear $DNA$ (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one $DNA$ bandwhile linear $DNA$ shows two fragments. Explain.

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$A$ : Restriction enzymes belong to a larger class of enzymes called nucleases.

$R$ : Each restriction enzyme recognises a specific palindromic nucleotide sequence in the $DNA$.

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Choose the correct pair from the following

medium

(NEET-2020)

For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

easy

The role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is

Solution

Similar Questions

The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$ ' and ' $Y$ genes:

A plasmid $DNA$ and a linear $DNA$ (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one $DNA$ bandwhile linear $DNA$ shows two fragments. Explain.

$A$ : Restriction enzymes belong to a larger class of enzymes called nucleases. $R$ : Each restriction enzyme recognises a specific palindromic nucleotide sequence in the $DNA$.

Choose the correct pair from the following

For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

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$A$ : Restriction enzymes belong to a larger class of enzymes called nucleases.

$R$ : Each restriction enzyme recognises a specific palindromic nucleotide sequence in the $DNA$.