A plasmid DNA and a linear DNA (both are of same size) have one site for a restriction endonuclease.

English

Gujarati

Hindi

9.Biotechnology Principals and Process

medium

A plasmid $DNA$ and a linear $DNA$ (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one $DNA$ bandwhile linear $DNA$ shows two fragments. Explain.

Option A

Option B

Option C

Option D

Solution

It is because plasmid is a circular $DNA$ molecule.

$\Rightarrow$ When cut with enzyme, it becomes linear but does not get fragmented. Whereas, a linear $DNA$ molecule gets cut into two fragments. Hence, a single $DNA$ band is observed for plasmid while two $DNA$ bands are observed for linear $DNA$ in agarose gel.

Standard 12

Biology

In gel electrophoresis, the separated bands of $DNA$ are cut out and extracted from the gel piece. This step is called

medium

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the $DNA$ is :

medium

(NEET-2021)

In Recombinant $DNA$ technology antibiotics are used

easy

(NEET-2020)

Plasmid are suitable vectors for gene cloning because

medium

Two enzymes responsible for restricting the growth of bacteriophages in $E .$ coli were isolated. One was methylase and other was restriction endonuclease. What is the significance of methylase?

medium

A plasmid $DNA$ and a linear $DNA$ (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one $DNA$ bandwhile linear $DNA$ shows two fragments. Explain.

Solution

Similar Questions

In gel electrophoresis, the separated bands of $DNA$ are cut out and extracted from the gel piece. This step is called

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the $DNA$ is :

In Recombinant $DNA$ technology antibiotics are used

Plasmid are suitable vectors for gene cloning because

Two enzymes responsible for restricting the growth of bacteriophages in $E .$ coli were isolated. One was methylase and other was restriction endonuclease. What is the significance of methylase?

Start a Free Trial Now