Two enzymes responsible for restricting growth of bacteriophages in E . coli were isolated. One was

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9.Biotechnology Principals and Process

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Two enzymes responsible for restricting the growth of bacteriophages in $E .$ coli were isolated. One was methylase and other was restriction endonuclease. What is the significance of methylase?

Protection of host $DNA$ from the action of restriction endonuclease by adding methyl group to one or two bases usually with in the sequence recognized by restriction enzyme

Able to ligate the two cohesive ends of $DNA$ molecule

Able to remove the methyl group and hence, prevent the action of restriction endonuclease on host $DNA$

Able to cut the $DNA$ of bacteriophage at specific sites

Solution

Protection of host $DNA$ from the action of restriction endonuclease by adding methyl group to one or two bases usually with in the sequence recognized by restriction enzyme

Standard 12

Biology