Manipulation of DNA in genetic engineering became possible due to discovery of

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9.Biotechnology Principals and Process

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Manipulation of $DNA$ in genetic engineering became possible due to the discovery of

Restriction endonuclease

$DNA$ ligase

Transcriptase

Primase

Solution

Isolation of restriction endonucleases by Nathans and Smith $(1970)$ made it possible to cut $DNA$ at specific sites. Restriction enzyme can cut both strands of $DNA$ when foreign nucleotides are introduced in the cell. They cleave $DNA$ to generate a nick with a $5 '$ phosphoryl and $3 '$ hydroxyl terminus.

Standard 12

Biology

The role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is

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A mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no $DNA $ bands were observed. What could be the reason ?

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$A$ : Selection of recombinants due to inactivation of antibiotics is cumbersome procedure.

$R $ : It requires simultaneous plating on two plates having different antibiotics.

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Which of the following is a plasmid ?

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Give types and functions of Nuclease Enzyme.

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Manipulation of $DNA$ in genetic engineering became possible due to the discovery of

Solution

Similar Questions

The role of $DNA$ ligase in the construction of a recombinant $DNA$ molecule is

A mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no $DNA $ bands were observed. What could be the reason ?

$A$ : Selection of recombinants due to inactivation of antibiotics is cumbersome procedure. $R $ : It requires simultaneous plating on two plates having different antibiotics.

Which of the following is a plasmid ?

Give types and functions of Nuclease Enzyme.

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$A$ : Selection of recombinants due to inactivation of antibiotics is cumbersome procedure.

$R $ : It requires simultaneous plating on two plates having different antibiotics.