Restriction enzymes that are used in construction of recombinant DNA are endonucleases which cut DNA

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9.Biotechnology Principals and Process

medium

Restriction enzymes that are used in the construction of recombinant $DNA$ are endonucleases which cut the $DNA$ at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the $DNA$ at specific- recognition sequence ?

Option A

Option B

Option C

Option D

Solution

If the restriction enzymes cuts the $DNA$ at random sites instead of at specific sites, then the $DNA$ fragments obtained will not have 'sticky ends'. construction of recombinant $DNA$ molecule would not be possible in the absence of these stick ends.

Standard 12

Biology