Assertion : Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.

Reason : A gene incorporated in the bacterial chromosomal genome-gets automatically transferred to the crop with which the bacterium is associated.

$A$ : Pst I generates single stranded over hanging stretches in $DNA$ after digestion that facilitate ligation.

$R$ : These short extensions can form phosphodiester bonds with their complementary counterparts.

Which of the following statements is correct in the context of observing $DNA$ separated by agarose gel electrophoresis?

Which of the following is not a restriction endonuclease?

Significance of treating bacterial cells with calcium chloride before transformation is to facilitate