Which of following statements is correct in context of observing DNA separated by agarose gel electr

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9.Biotechnology Principals and Process

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Which of the following statements is correct in the context of observing $DNA$ separated by agarose gel electrophoresis?

$DNA$ can be seen in visible light

$DNA$ can be seen without staining in visible light

Ethidium bromide stained $DNA$ can be seen in visible light

Ethidium bromide stained $DNA$ can be seen under exposure to $UV$ light

Solution

Ethidium bromide is a molecule that becomes intercalated between the stacked bases of the $DNA$ molecule. Soaking a $DNA$ containing gel in ethidium bromide will result in concentration of chemical within the $DNA$. Illumination of the soaked gel with light in the $UV$ range ($260 -300$ nm) results in fluorescence of ethidium bromide and the $DNA$ shows up on the gel as a band of fluorescence.

Standard 12

Biology

$A$ – amp$^R$ has recognition sites for Pvu $I$.

$R$ – tet$^R$ gene has not recognition site for Hind $III$.

medium

During 'gene cloning' which is called a gene taxi?

medium

Which of the following statements does not hold true for restriction enzyme?

medium

In Recombinant $DNA$ technology antibiotics are used

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(NEET-2020)

Following enzymes/chemical/technique are used in the process of gel electrophoresis

$I.$ sample $DNA$ is cut into fragments

$II.$ restriction endonucleases

$III.$ agarose gel

$IV.$ ethidium bromide

$V.$ UV-radiation

$VI.$ elution

Mark the correct sequence of their use

medium

Which of the following statements is correct in the context of observing $DNA$ separated by agarose gel electrophoresis?

Solution

Similar Questions

$A$ – amp$^R$ has recognition sites for Pvu $I$. $R$ – tet$^R$ gene has not recognition site for Hind $III$.

During 'gene cloning' which is called a gene taxi?

Which of the following statements does not hold true for restriction enzyme?

In Recombinant $DNA$ technology antibiotics are used

Following enzymes/chemical/technique are used in the process of gel electrophoresis $I.$ sample $DNA$ is cut into fragments $II.$ restriction endonucleases $III.$ agarose gel $IV.$ ethidium bromide $V.$ UV-radiation $VI.$ elution Mark the correct sequence of their use

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$A$ – amp$^R$ has recognition sites for Pvu $I$.

$R$ – tet$^R$ gene has not recognition site for Hind $III$.

Following enzymes/chemical/technique are used in the process of gel electrophoresis

$I.$ sample $DNA$ is cut into fragments

$II.$ restriction endonucleases

$III.$ agarose gel

$IV.$ ethidium bromide

$V.$ UV-radiation

$VI.$ elution

Mark the correct sequence of their use