Write short note on Biochemical characterisation of Transforming Principle.
Write short note on Biochemical characterisation of Transforming Principle.
Prior to the work of Oswald Avery, Colin MacLeod and Maclyn McCarty ($1933$-$44$) the genetic material was thought to be a protein.
They worked to determine the biochemical nature of 'transforming principle' in Griffith's experiment.
They purified biochemicals (proteins, $DNA$, $RNA$ etc.) from the heat-killed $S$ cells to see which ones could transform live $\mathrm{R}$ cells into $\mathrm{S}$ cells.
The discovered that $DNA$ alone from $S$ becteria caused $\mathrm{R}$ bacteria to become transformed.
They also discovered that protein digesting enzymes (proteases) and $RNA$ - digesting enzymes [$RNases$] did not affect transformation, so the transforming substance was not a protein or $RNA$.
Digestion with $DNase$ did inhibit transformation suggesting that the $DNA$ caused the transformation.
They concluded that $DNA$ is the hereditary material, but not all biologists were convinced.
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A molecule that can act as a genetic material must fulfill the traits given below, except
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- [NEET 2016]
Which of the following was used by Hershey and Chase to prove that $DNA$ is the chemical basis of heredity
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- [AIIMS 1993]
The scientists involved in discovery of $DNA$ as chemical basis of heredity were
The scientists involved in discovery of $DNA$ as chemical basis of heredity were